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rabbit polyclonal antibodies against smct1 and mpc1  (Novus Biologicals)


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    Novus Biologicals rabbit polyclonal antibodies against smct1 and mpc1
    MCT family-member and macropinocytosis activities critically regulate bladder cancer cell susceptibility to 3-BrPA - the dispensable role of MPC components. ( a ) Representative (three independent experiments) Western blotting profiles of whole-cell protein extracts obtained from RT4 and T24 cells, seeded at ~60 % confluency and treated with the indicated doses of 3-BrPA for 24 h. The proteins examined were MCT1, MCT4, SMCT1, <t>MPC1</t> and MPC2, while Actin was used as molecule of reference. ( b ) Representative (three independent experiments) immunofluorescence images of MCT1 expression and localization in RT4 and T24 cells, seeded at ~60 % confluency and exposed to the indicated doses of 3-BrPA for 4 h (also, see Fig. ). Scale bars: 3 μm. ( c - e ) MTT cytotoxicity assays of T24 cells, grown at low (and ~60 %; data not shown) confluency and treated with the indicated doses of 3-BrPA for 24 h, in the absence or presence (pre-incubation for the denoted time) of 1 or 10 μM UK-5099 ( c ), 0.1 or 1 μM AR-C155858 ( d ) and 10 or 50 μM EIPA ( e ) (both low and ~60 % confluency allowed the striking survival of 3-BrPA-treated T24 cells grown in the presence of either AR-C155858 or EIPA, but not UK-5099, inhibitor). Each inhibitor remained in the growth medium with half of its initial respective concentration(s) for 24 h more, post-pre-incubation. Survival rates of each cocktail (3-BrPA plus inhibitor) were normalized according to the respective values of inhibitor only. Stock solutions of all three inhibitors (UK-5099, AR-C155858 and EIPA) ( c - e ) were prepared in DMSO. Pre-incubation (for 1.5 h) of T24 with 100 μM EIPA proved significantly detrimental for the cells (data not shown). ( c - e ) Results are reported as mean ± standard deviation of triplicates of three independent experiments. * P < 0.001
    Rabbit Polyclonal Antibodies Against Smct1 And Mpc1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against smct1 and mpc1/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibodies against smct1 and mpc1 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants"

    Article Title: 3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants

    Journal: Molecular Cancer

    doi: 10.1186/s12943-015-0399-9

    MCT family-member and macropinocytosis activities critically regulate bladder cancer cell susceptibility to 3-BrPA - the dispensable role of MPC components. ( a ) Representative (three independent experiments) Western blotting profiles of whole-cell protein extracts obtained from RT4 and T24 cells, seeded at ~60 % confluency and treated with the indicated doses of 3-BrPA for 24 h. The proteins examined were MCT1, MCT4, SMCT1, MPC1 and MPC2, while Actin was used as molecule of reference. ( b ) Representative (three independent experiments) immunofluorescence images of MCT1 expression and localization in RT4 and T24 cells, seeded at ~60 % confluency and exposed to the indicated doses of 3-BrPA for 4 h (also, see Fig. ). Scale bars: 3 μm. ( c - e ) MTT cytotoxicity assays of T24 cells, grown at low (and ~60 %; data not shown) confluency and treated with the indicated doses of 3-BrPA for 24 h, in the absence or presence (pre-incubation for the denoted time) of 1 or 10 μM UK-5099 ( c ), 0.1 or 1 μM AR-C155858 ( d ) and 10 or 50 μM EIPA ( e ) (both low and ~60 % confluency allowed the striking survival of 3-BrPA-treated T24 cells grown in the presence of either AR-C155858 or EIPA, but not UK-5099, inhibitor). Each inhibitor remained in the growth medium with half of its initial respective concentration(s) for 24 h more, post-pre-incubation. Survival rates of each cocktail (3-BrPA plus inhibitor) were normalized according to the respective values of inhibitor only. Stock solutions of all three inhibitors (UK-5099, AR-C155858 and EIPA) ( c - e ) were prepared in DMSO. Pre-incubation (for 1.5 h) of T24 with 100 μM EIPA proved significantly detrimental for the cells (data not shown). ( c - e ) Results are reported as mean ± standard deviation of triplicates of three independent experiments. * P < 0.001
    Figure Legend Snippet: MCT family-member and macropinocytosis activities critically regulate bladder cancer cell susceptibility to 3-BrPA - the dispensable role of MPC components. ( a ) Representative (three independent experiments) Western blotting profiles of whole-cell protein extracts obtained from RT4 and T24 cells, seeded at ~60 % confluency and treated with the indicated doses of 3-BrPA for 24 h. The proteins examined were MCT1, MCT4, SMCT1, MPC1 and MPC2, while Actin was used as molecule of reference. ( b ) Representative (three independent experiments) immunofluorescence images of MCT1 expression and localization in RT4 and T24 cells, seeded at ~60 % confluency and exposed to the indicated doses of 3-BrPA for 4 h (also, see Fig. ). Scale bars: 3 μm. ( c - e ) MTT cytotoxicity assays of T24 cells, grown at low (and ~60 %; data not shown) confluency and treated with the indicated doses of 3-BrPA for 24 h, in the absence or presence (pre-incubation for the denoted time) of 1 or 10 μM UK-5099 ( c ), 0.1 or 1 μM AR-C155858 ( d ) and 10 or 50 μM EIPA ( e ) (both low and ~60 % confluency allowed the striking survival of 3-BrPA-treated T24 cells grown in the presence of either AR-C155858 or EIPA, but not UK-5099, inhibitor). Each inhibitor remained in the growth medium with half of its initial respective concentration(s) for 24 h more, post-pre-incubation. Survival rates of each cocktail (3-BrPA plus inhibitor) were normalized according to the respective values of inhibitor only. Stock solutions of all three inhibitors (UK-5099, AR-C155858 and EIPA) ( c - e ) were prepared in DMSO. Pre-incubation (for 1.5 h) of T24 with 100 μM EIPA proved significantly detrimental for the cells (data not shown). ( c - e ) Results are reported as mean ± standard deviation of triplicates of three independent experiments. * P < 0.001

    Techniques Used: Western Blot, Immunofluorescence, Expressing, Incubation, Concentration Assay, Standard Deviation



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    rabbit polyclonal antibodies against smct1 and mpc1  (Novus Biologicals)
    90
    Novus Biologicals rabbit polyclonal antibodies against smct1 and mpc1
    MCT family-member and macropinocytosis activities critically regulate bladder cancer cell susceptibility to 3-BrPA - the dispensable role of MPC components. ( a ) Representative (three independent experiments) Western blotting profiles of whole-cell protein extracts obtained from RT4 and T24 cells, seeded at ~60 % confluency and treated with the indicated doses of 3-BrPA for 24 h. The proteins examined were MCT1, MCT4, SMCT1, <t>MPC1</t> and MPC2, while Actin was used as molecule of reference. ( b ) Representative (three independent experiments) immunofluorescence images of MCT1 expression and localization in RT4 and T24 cells, seeded at ~60 % confluency and exposed to the indicated doses of 3-BrPA for 4 h (also, see Fig. ). Scale bars: 3 μm. ( c - e ) MTT cytotoxicity assays of T24 cells, grown at low (and ~60 %; data not shown) confluency and treated with the indicated doses of 3-BrPA for 24 h, in the absence or presence (pre-incubation for the denoted time) of 1 or 10 μM UK-5099 ( c ), 0.1 or 1 μM AR-C155858 ( d ) and 10 or 50 μM EIPA ( e ) (both low and ~60 % confluency allowed the striking survival of 3-BrPA-treated T24 cells grown in the presence of either AR-C155858 or EIPA, but not UK-5099, inhibitor). Each inhibitor remained in the growth medium with half of its initial respective concentration(s) for 24 h more, post-pre-incubation. Survival rates of each cocktail (3-BrPA plus inhibitor) were normalized according to the respective values of inhibitor only. Stock solutions of all three inhibitors (UK-5099, AR-C155858 and EIPA) ( c - e ) were prepared in DMSO. Pre-incubation (for 1.5 h) of T24 with 100 μM EIPA proved significantly detrimental for the cells (data not shown). ( c - e ) Results are reported as mean ± standard deviation of triplicates of three independent experiments. * P < 0.001
    Rabbit Polyclonal Antibodies Against Smct1 And Mpc1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against smct1 and mpc1/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal antibodies against smct1 and mpc1 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    MCT family-member and macropinocytosis activities critically regulate bladder cancer cell susceptibility to 3-BrPA - the dispensable role of MPC components. ( a ) Representative (three independent experiments) Western blotting profiles of whole-cell protein extracts obtained from RT4 and T24 cells, seeded at ~60 % confluency and treated with the indicated doses of 3-BrPA for 24 h. The proteins examined were MCT1, MCT4, SMCT1, MPC1 and MPC2, while Actin was used as molecule of reference. ( b ) Representative (three independent experiments) immunofluorescence images of MCT1 expression and localization in RT4 and T24 cells, seeded at ~60 % confluency and exposed to the indicated doses of 3-BrPA for 4 h (also, see Fig. ). Scale bars: 3 μm. ( c - e ) MTT cytotoxicity assays of T24 cells, grown at low (and ~60 %; data not shown) confluency and treated with the indicated doses of 3-BrPA for 24 h, in the absence or presence (pre-incubation for the denoted time) of 1 or 10 μM UK-5099 ( c ), 0.1 or 1 μM AR-C155858 ( d ) and 10 or 50 μM EIPA ( e ) (both low and ~60 % confluency allowed the striking survival of 3-BrPA-treated T24 cells grown in the presence of either AR-C155858 or EIPA, but not UK-5099, inhibitor). Each inhibitor remained in the growth medium with half of its initial respective concentration(s) for 24 h more, post-pre-incubation. Survival rates of each cocktail (3-BrPA plus inhibitor) were normalized according to the respective values of inhibitor only. Stock solutions of all three inhibitors (UK-5099, AR-C155858 and EIPA) ( c - e ) were prepared in DMSO. Pre-incubation (for 1.5 h) of T24 with 100 μM EIPA proved significantly detrimental for the cells (data not shown). ( c - e ) Results are reported as mean ± standard deviation of triplicates of three independent experiments. * P < 0.001

    Journal: Molecular Cancer

    Article Title: 3-BrPA eliminates human bladder cancer cells with highly oncogenic signatures via engagement of specific death programs and perturbation of multiple signaling and metabolic determinants

    doi: 10.1186/s12943-015-0399-9

    Figure Lengend Snippet: MCT family-member and macropinocytosis activities critically regulate bladder cancer cell susceptibility to 3-BrPA - the dispensable role of MPC components. ( a ) Representative (three independent experiments) Western blotting profiles of whole-cell protein extracts obtained from RT4 and T24 cells, seeded at ~60 % confluency and treated with the indicated doses of 3-BrPA for 24 h. The proteins examined were MCT1, MCT4, SMCT1, MPC1 and MPC2, while Actin was used as molecule of reference. ( b ) Representative (three independent experiments) immunofluorescence images of MCT1 expression and localization in RT4 and T24 cells, seeded at ~60 % confluency and exposed to the indicated doses of 3-BrPA for 4 h (also, see Fig. ). Scale bars: 3 μm. ( c - e ) MTT cytotoxicity assays of T24 cells, grown at low (and ~60 %; data not shown) confluency and treated with the indicated doses of 3-BrPA for 24 h, in the absence or presence (pre-incubation for the denoted time) of 1 or 10 μM UK-5099 ( c ), 0.1 or 1 μM AR-C155858 ( d ) and 10 or 50 μM EIPA ( e ) (both low and ~60 % confluency allowed the striking survival of 3-BrPA-treated T24 cells grown in the presence of either AR-C155858 or EIPA, but not UK-5099, inhibitor). Each inhibitor remained in the growth medium with half of its initial respective concentration(s) for 24 h more, post-pre-incubation. Survival rates of each cocktail (3-BrPA plus inhibitor) were normalized according to the respective values of inhibitor only. Stock solutions of all three inhibitors (UK-5099, AR-C155858 and EIPA) ( c - e ) were prepared in DMSO. Pre-incubation (for 1.5 h) of T24 with 100 μM EIPA proved significantly detrimental for the cells (data not shown). ( c - e ) Results are reported as mean ± standard deviation of triplicates of three independent experiments. * P < 0.001

    Article Snippet: Rabbit polyclonal antibodies against SMCT1 and MPC1 were purchased from Novus Biologicals LLC (Connecticut, USA).

    Techniques: Western Blot, Immunofluorescence, Expressing, Incubation, Concentration Assay, Standard Deviation